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In PCR, we need to determine the optimal annealing temperature, which is ~5C lower than Tm.? Why ?Please help

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  • In PCR, we need to determine the optimal annealing temperature, which is ~5C lower than Tm.? Why ?Please help


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In PCR, we need to determine the optimal annealing temperature, which is ~5C lower than Tm.? Why ... we need help. Please keep ... appropriate annealing ...
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Positive: 86 %
What is the annealing temperature ... to determine the optimal annealing temperature of ... we design primers we set Tm with the PCR ...
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Positive: 83 %

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The Simple Fool’s Guide to PCR , ... other workers may have solved ones with which we need help. Please keep in ... Weigh each sample and determine the ...
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Positive: 86 %
... we're here to help. ... the stringency of primer annealing. At lower temperatures, PCR primers can ... need to be varied to determine the optimal ...
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Positive: 81 %
QUANTITATIVE RT-PCR ... the PCR to determine the optimal ... the annealing temperature (2 degrees lower than calculated Tm) for 1
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Positive: 67 %
Real-time PCR (qPCR) primer design using free ... Optimal real-time PCR annealing temperatures are ... have a lower melting temperature (T m) than the ...
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Positive: 44 %

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